Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human & Mouse Phospho-PLC-γ1 (Tyr1253) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human & Mouse Phospho-PLC-γ1 (Tyr1253) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total PLK1 Detection Kit, 500 Assay Points
HTRF Human Total PLK1 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human and mouse Total STAT5 Detection Kit, 10,000 Assay Points
HTRF Human and mouse Total STAT5 Detection Kit, 10,000 Assay Points
Perkin Elmer US LLC Hangdown Wire Alignment Tool
This tool ships with the TGA 8000 Spares Kit and the PYRIS 1 TGA with Autosampler Spares Kit.
Molecular Devices Time Resolved Filter, 642 nm, 10 nm Bandwidth
Manufactured by Molecular Devices, this time-resolved interference filter provides a narrow optical band centered at 642 nm with a 10 nm full width at half maximum (FWHM) for improved signal specificity in time-resolved fluorescence assays. It is intended as a plug-in accessory for compatible multi-mode microplate readers to enable sensitive TRF measurements.
- Center wavelength: 642 nm.
- Bandwidth: 10 nm FWHM.
- Filter type: time-resolved fluorescence interference filter.
- Optimized for time-resolved fluorescence assays to reduce background signal.
- Designed as a plug-in accessory for compatible multi-mode microplate readers.
Revvity Health Sciences Inc HTRF Human & Mouse Total p300 Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Total p300 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-JAK1 (Tyr1034/1035) Detection Kit, 500 Assay Points
HTRF Human Phospho-JAK1 (Tyr1034/1035) Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total IκKα Detection Kit, 50,000 Assay Points
HTRF Human Total IκKα Detection Kit, 50,000 Assay Points
Molecular Devices Fluorescence Polarization Filter, 595 nm, 35 nm BW
A fluorescence polarization emission filter optimized for 595 nm detection with a 35 nm bandwidth and vertical polarization. Designed for fluorescence polarization assays, the filter improves signal selectivity and reduces background when detecting TAMRA-like red fluorophores. It is compatible with filter-based microplate readers.
- Center wavelength 595 nm for red emission detection.
- 35 nm bandwidth to balance sensitivity and selectivity.
- Vertical polarization for fluorescence polarization measurements.
- Suitable for use in filter-based microplate reader filter holders.
- Optimized for TAMRA and similar red fluorophores.
Revvity Health Sciences Inc HTRF Human IL-2 High Performance Detection Kit, 10,000 Assay Points
HTRF Human IL-2 High Performance Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human & Mouse Total Raf1 Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human & Mouse Total Raf1 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human and Mouse Total IKZF1, Control Lysate
HTRF Human and Mouse Total IKZF1, Control Lysate
Enterprise Technology Solutions WetTask Customizable Wet Wipin
WetTask Customizable Wet Wiping System Bucket White/Blue 4/Carton
Revvity Health Sciences Inc HTRF Phospho-Total protein lysis buffer 6, 130 mL
HTRF Phospho-Total protein lysis buffer 6, 130 mL
Precision Glassblowing of Colorado TJA INJECTOR 1.0MM 12540701
Quartz Injector, 1.0mm (2 red rings), for TJA 61E, Iris & Intrepid Radial, and for TJA 61E Trace, Iris AP, & Intrepid